FIGURE 11.

Inability of ELK3 to interact with AR. A and B, HeLa cells plated in hormone-depleted medium were transfected with (ELK1)₂-TATA-Luc. The same amount of either the ELK1 or ELK3 expression plasmid or the pCMV vector control plasmid was co-transfected. In all cases, the AR expression plasmid was co-transfected. The cells were treated with vehicle (Veh) or testosterone (Test) (10 nm) and harvested 48 h after transfection to measure luciferase activity (A) or to measure relative mRNA levels of ELK1 and ELK3 (B). C, LNCaP cells plated in hormone-depleted medium were infected with either ELK1 shRNA or control (Ctrl) shRNA lentivirus along with pCDH empty vector lentivirus or pCDH-ELK3 lentivirus. 72 h after infection, cells were harvested. The expression levels of mRNAs for ELK3 (top panel) and ELK1 (bottom panel) were measured by quantitative real time PCR. D, LNCaP cells plated in hormone-depleted medium were infected with either ELK1 shRNA or control shRNA lentivirus along with pCDH empty vector lentivirus or pCDH-ELK3 lentivirus. 72 h later, the cells were treated with either vehicle or R1881 (1 nm) for 48 h and harvested. The expression levels of mRNAs for the indicated genes were measured by quantitative real time PCR. * and **, p < 0.001. Error bars represent S.D.