FIGURE 5.

Lack of dependence on SRF for ELK1-dependent gene activation by androgen. A, hormone-depleted LNCaP cells were infected with SRF shRNA (sh) or control (Ctrl) shRNA lentivirus. 72 h later (after the knockdown had occurred), cells were treated with vehicle or R1881 (1 nm). 48 h after treatment, cells were harvested for RNA and protein. SRF mRNA expression was measured using quantitative real time PCR, and the cell lysates were analyzed by Western blot for SRF with GAPDH as loading control (inset). B, the RNA samples from A were used to measure mRNA levels of PSA and TMPRSS2 by quantitative real time PCR. C, the RNA samples used in A were analyzed for expression of the indicated genes by quantitative real time PCR. *, p < 0.001. Error bars represent S.D.