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. 2013 Feb 26;288(16):11024–11037. doi: 10.1074/jbc.M112.436006

FIGURE 2.

FIGURE 2.

ATPZs promote intramolecular cysteine disulfide formation in 4-R Tau. A, K18PL was incubated for 1 h at 37 °C in the absence (−) or presence of CNDR-51348 (51348) or CNDR-51449 (51449) or was pre-reduced (R) or pre-oxidized (O). The samples were then analyzed for IAA-OG reactivity. Both the IAA-OG fluorescence and corresponding Coomassie blue staining are shown for each IAA-OG-treated sample after SDS-PAGE. B, K18PL, K18, or full-length 4-R Tau (T40) were incubated for 1 h at 37 °C in the absence (−) or presence of CNDR-51348 (51348) or CNDR-51449 (51449) or were pre-reduced (R) or pre-oxidized (O) followed by native gel electrophoresis. C, K18PL in which both cysteines were mutated to alanine (2xCA) was incubated for 1 h at 37 °C in the absence (2xCA) or presence of CNDR-51348 (2xCA:51348) followed by SEC analysis.