FIGURE 5.

The purified CarCDE complex catalyzes reduction of ferredoxin and caffeyl-CoA simultaneously. The assay buffer (buffer B) contained 0.2 mm NADH, 5 μm caffeyl-CoA, 8 μg of the purified CarCDE complex, and the caffeyl-CoA regeneration system (200 units of the CoA transferase CarA and 0.2 mm caffeate). The reaction was started by the addition of 20 μm ferredoxin. Reduction of caffeyl-CoA (dashed line; monitored at 312 nm via decline of caffeate) and reduction of ferredoxin (solid line; monitored at 430 nm) were monitored simultaneously.