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The purified CarCDE complex reduces ferredoxin and caffeyl-CoA in stoichiometric amounts. The reduction of caffeyl-CoA and ferredoxin was monitored simultaneously (see Fig. 5). Data points from 5-s intervals were taken to calculate the amount of reduced electron carrier from the absorbance difference and the molar extinction coefficient. The amount of reduced caffeyl-CoA is plotted against the amount of reduced ferredoxin.
