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. 2013 Mar 7;288(16):11287–11293. doi: 10.1074/jbc.M112.435842

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — PafA pupylation of titin-I27 variants. A, pupylation of titin-I27 variants (15 μm each) by PafA (0.5 μm) and Fl-Pup^E (1 μm). The inset demonstrates an I27₁ pupylation reaction with PafA (0.5 μm) and Fl-Pup^E (7 μm) that continued to completion. B, steady-state pupylation rates by PafA (0.5 μm) and Fl-Pup^E (15 μm) at increasing concentrations of titin-I27 variants. The curves are fitted to the Hill equation. The averages ± S.D. (error bars) of three experiments are presented.