TABLE 1.

Steady-state kinetic parameters for the PafA-catalyzed reactions measured in this work

Substrate	Vmax	K0.5	Hill coefficient
	min−1/PafA	μm
PanB	1.4 ± 0.1a	21 ± 2	1.9 ± 0.3
I271	1.4 ± 0.1a	534 ± 87	1.6 ± 0.2
I272	1.4 ± 0.1a	348 ± 42	1.3 ± 0.1
I273	1.3 ± 0.1a	368 ± 45	1.2 ± 0.08
Pup	0.14 ± 0.01	1.3 ± 0.4	0.94 ± 0.2

^a The values are equivalent to the k_cat of the reactions, as measurements were carried out at saturating Pup^E concentrations.