TABLE 5.

M. smegmatis cytosolic and secreted fraction metal levels

Metala	Cytosolic fraction			Secreted fraction
	Wild type	ctpC::hyg	Complemented	Wild type	ctpC::hyg	Complemented
	pmol/mg protein			pmol/mg protein
Manganese	7.1 ± 0.9	20 ± 1	12 ± 2	38 ± 3	20 ± 4	41 ± 2
Zinc	596 ± 17	519 ± 47	492 ± 67	182 ± 13	162 ± 24	168 ± 34
Copper	8 ± 2	4 ± 1	9 ± 3	22 ± 4	14 ± 2	27 ± 3
Iron	489 ± 24	457 ± 59	480 ± 43	629 ± 36	784 ± 45	617 ± 28

^a Cells were cultured on Sauton's medium supplemented with 50 μm Mn²⁺.