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Lipid rafts in CNF1-induced NF-κB activation. (A) Graph showing the inhibitory effect of filipin on the CNF1-induced p65 nuclear translocation. ▪, control cells; □, filipin-treated cells. (B) Confocal microscopy analysis of HEp-2 control (-CNF1) and cells treated for 4 h with CNF1 (+CNF1) stained with anti-caveolin-1 (left) or anti-IkBα (right) antibodies. Note that IkBα colocalizes with caveolin-1 at the level of ruffles. Bar, 10 μm.
