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. 2013 Mar 5;288(16):11004–11012. doi: 10.1074/jbc.M112.441816

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Sirtuin modulators regulate keratinocytes motility. A, Western blot analysis of histone H4 acetylated lysine 16 (H4K16Ac) and histone H3 acetylated lysine 14 (H3K14Ac) in the presence of Resv, MC2562, and solvent. Relative densitometry is shown in the right panel. B, SIRT1 activity assay performed in HaCaT cells treated with Resv, MC2562, or Sirtinol. DMSO was used as a control solvent. *, p < 0.05 versus solvent. C, phase contrast panels showing an in vitro scratch assay after 24-h treatment with SIRT modulators in HaCaT cells. 10% FCS condition represents a positive control. D, percentage of closure quantification in the in vitro scratch assay. *, °, and #, p < 0.05 versus solvent.