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GFP-CEN binds kinetochores, centromeres, and spindle poles without disrupting global spindle morphology. PtK2 cells in prophase were injected with GFP′ control (A) or GFP-CEN (B), the centromere dominant-negative version of MCAK. At 30 min postinjection, cells were fixed and immunostained for MTs and DNA. Bars, 10 μm.
