Table 1.
Measurements of MCAK versus CREST localization at the centromere/kinetochore region
| Mitotic stage | Interkinetochore distance of CREST | Length of MCAK staining at centromeres |
|---|---|---|
| avg. μm ± SEM (n) | avg. μm ± SEM (n) | |
| Prophase | 1.34 ± 0.06 (22) | 1.43 ± 0.06 (22) |
| Prometaphase | ||
| Chrom at the pole | 2.30 ± 0.13 (17) | 2.31 ± 0.13 (17) |
| Bioriented chrom | 3.27 ± 0.08 (77) | 3.11 ± 0.09 (77) |
| Metaphase | 3.81 ± 0.13 (27) | 3.55 ± 0.14 (27) |
| Anaphase A | NA | 1.23 ± 0.04 (53) |
chrom, chromosomes; NA, not applicable.
Interkinetochore distances were determined by measuring the distance from the outer edge of CREST staining at one kinetochore to outer edge of CREST staining at its sister kinetochore versus the length of MCAK staining at centromeres. Sample sizes for all interkinetochore measurements are given in number of chromosomes.