Table 4.
Analysis of chromosome behavior in GFP′ versus GFP-CEN–injected cells
| Measurement | Control GFP'-injected cells | GFP-CEN–injected cells | Student's t test | Trends |
|---|---|---|---|---|
| avg. ± SEM (n) | avg ± SEM (n) | |||
| Antipoleward rate during congression (μm/min) | 1.69 ± 0.16 (23) | 1.64 ± 0.09 (33) | p = 0.76 | No change |
| Poleward rate during congression (μm/min) | 1.76 ± 0.13 (23) | 1.72 ± 0.16 (33) | p = 0.95 | No change |
| Time in antipoleward motion during congression (%) | 96.5 ± 2.0 (23) | 80.9 ± 3.0 (33) | p < 0.001 | 12% decrease |
| Time in poleward motion during congression (%) | 1.0 ± 0.1 (23) | 10.3 ± 2.4 (33) | p < 0.001 | 10-fold increase |
| Oscillation rate at the equator (μm/min) | 1.55 ± 0.21 (10) | 1.79 ± 0.19 (13) | p = 0.08 | No change |
| No. switches between AP and P movement during oscillations | 2.6 ± 0.4 (10) | 4.2 ± 0.5 (13) | p < 0.05 | 63% increase |
| Chromatid mass anaphase A segregation rate (μm/min) | 2.06 ± 0.19 (22) | 2.05 ± 0.14 (30) | p =0.32 | No change |
| Chromatid mass anaphase B segregation rate (μm/min) | 1.22 ± 0.10 (22) | 1.12 ± 0.07 (30) | p < 0.05 | 8% decrease |
| Pole-pole anaphase A separation rate (μm/min) | 0.70 ± 0.09 (22) | 0.63 ± 0.11 (30) | p = 0.50 | No change |
| Pole-pole anaphase B separation rate (μm/min) | 1.00 ± 0.09 (22) | 0.80 ± 0.07 (30) | p < 0.01 | 20% decrease |
Chromosome behavior was analyzed in cells injected with control GFP' fusion protein or GFP-CEN according to MATERIALS AND METHODS. Sample sizes for all congression and metaphase oscillation measurements are given in number of chromosomes. Sample sizes for anaphase measurements are given in number of chromatid masses or number of spindle poles analyzed.