FIG. 2.

Magnitude of AAV capsid-specific T cell responses. (A) Average numbers of AAV capsid-specific T cells over 10⁶ live CD3 cells for all cohorts. As indicated in the legend, IS patients before and after IS treatment initiation are shown in blue and red, respectively, the two consecutively harvested samples from healthy humans are shown in yellow and purple, respectively, and samples from comorbid control subjects are shown in light green. (B) Percentages of individuals who failed to respond (red; fewer than 20 cells/10⁶ live CD3⁺ cells of a given subset), showed low responsiveness (yellow; 20 to <100 cells/10⁶ live CD3⁺ cells), intermediate responsiveness (green; 100 to <1000 cells/10⁶ live CD3⁺ cells), and high responsiveness (blue; ≤1000 cells/10⁶ live CD3⁺ cells). Normalized background values were subtracted before the analysis. (C) Percentages of transplant patients who, after IS, either maintained stable numbers of AAV capsid-specific T cells (less than 2-fold changes, white part of columns), showed more that 2-fold decreases (gray part of columns), or increases (black part of columns). (D) AAV capsid-specific CD4⁺ cells (top) or CD8⁺ cells (bottom) and their subsets of individual patients before (left) and after (right) IS. Overall responses are shown in green, effector cells in blue, effector memory cells in red, and central memory cells in yellow.