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Reporter expression from D-MEF2 mutated IRE fragments
Reporter expression levels (0–3) from IRE fragments where the D-MEF2 binding sites have been mutated (black) or not (white). The mutated versions were as follows: site 1: TTAAAAATAC → TTAAGCCTAC; site 2: TTAAAAATAA → TTAAGCCTAA; and site 3 CTAAAAATG → CTAAGCCTG. Fragments extent is indicated in kb and ordered with respect to the promoter (to the right).