Fig. 2.

Expression analysis of selective J-proteins of rice by semiquantitative RT-PCR. a For RT-PCR cDNA used was synthesized using 5 μg RNA isolated from rice seedlings stressed as follows: HS1 heat shock at 42 °C for 10 min, HS2 heat shock at 42 °C for 60 min, ABA 100 μM abcissic acid for 3 h, CS cold stress at 6 °C for 6 h, SS salt stress in 150 mM NaCl for 6 h. Con unstressed control maintained at 26 °C. bIn silico expression analysis of types A, B, and D J-proteins of rice under stress conditions. Expression profiles of type C J-proteins are presented in ESM Fig. 4. Primers used for RT-PCR are listed in ESM Table 2