Fig. 2.

Effect of different concentrations of GRP78 and time-course of tunicamycin action in high glucose culture DMEM. aGRP78 expression by western blotting (left to right control; 5 μg/mL TM for 6 h; 5 μg/mL TM for 12 h; 5 μg/mL TM for 24 h; 1 μg/mL TM for 6 h; 1 μg/mL TM for 12 h; 1 μg/mL TM for 24 h). b GAPDH expression by Western blotting. c Relative expression of GRP78 at different times and concentration. It was similar at different times and concentrations