Fig. 4.

Bone marrow neutrophils were isolated and sorted based on GR-1⁺/CD11b⁺ staining by BD FACSAria sorting, which enriched the neutrophil population from 53 % to 91 % (a). This concentrated neutrophil population was used to determine β1-AR mRNA expression (b) in reference to BALB/c (lanes 2 & 4) and β1β2KO (lanes 3 & 5) neutrophil (lanes 2 & 3) and spleen (lanes 4 & 5) preparations. Lane 1 contained nucleotide bp control markers and lane 6 was a negative control. PCR was performed on the same mice to ensure they expressed the proper knock-out genes (c); lanes 1, 2 and 3 represent BALB/c cells, β1β2KO cells, and negative control, respectively