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Expression of β1-AR by peripheral blood leukocytes. After RBC lysis, cells were washed, fixed, and permeabilized. Based on flow cytometric separation of blood leukocytes with light scatter and antigens (see Materials and methods), the permeabilized subpopulations were assayed for the amount of β1-AR (Geomean of Fluroescence Intensity) with rabbit IgG or rabbit polyclonal antibodies to the intracellular cytoplasmic domain of β1-AR followed by FITC-goat anti-rabbit IgG
