Fig. 3.

Hsp70 gel application induces elevation of gamma–interferon production and cytotoxic response. a γ–IFN level in medium of splenocytes isolated from control animals, and animals treated with vehicle and with Hsp70 gel on days 14 and 20 after B16 tumor injection; b cytotoxic activity was measured using splenocytes derived on day 14 from animals as indicated in 3A and with the aid of standard protocol. Cell death was determined with the help of Cytotox-96 assay (see the text for details)