Figure 5.

Relative protein concentrations of total and phosphorylated mTOR at Ser²⁴⁴⁸ and Ser²⁴⁸¹ and calculated phospho-mTOR (Ser²⁴⁴⁸)/total mTOR and phosphor-mTOR (Ser²⁴⁸¹)/total mTOR ratios in M. quadriceps femoris of rats fed either a control diet (0 mg carnitine/kg diet; Control) or a diet supplemented with 1250 mg carnitine/kg diet (Carnitine). (A) Representative immunoblots specific to total mTOR, phosphor-mTOR (Ser²⁴⁴⁸), phosphor-mTOR (Ser²⁴⁸¹) and α-Tubulin as internal control are shown for one animal per group; immunoblots for the other animals revealed similar results. (B) Bars represent data from densitometric analysis and represent means ± SD (n = 6/group); bars are expressed relative to the protein level of the control group (= 1.00). ^* indicates a significant difference to the control group (P < 0.05).