Table 1. Comparison of protein expression levels in hibernation and active states of Myotis ricketti.
IDa | Accession numberb | Protein Identification (s) | MW/pIc | MPNd | PCe (%) | FCf(H/A) |
1 | Q6FG96 | 60S ribosomal protein P2, RPLP2 proteing | 11657.85/4.38 | 9 | 48.70 | - |
2 | Q6FG96 | 60S ribosomal protein P2, RPLP2 proteing | 11657.85/4.38 | 9 | 48.70 | ↓1.25 |
3 | P02608 | Myosin regulatory light chain 2, skeletal muscle isoform type 2 | 18895.34/4.79 | 24 | 99.00 | ↓1.45* |
4 | P60661 | Myosin, light polypeptide 6 | 16798.86/4.56 | 7 | 20.53 | ↓1.29 |
5 | P67936 | Tropomyosin alpha-4 chain | 28390.62/4.67 | 77 | 99.00 | ↓1.72** |
6 | P53505 | Actin, cytoplasmic type 5g | 41849.89/5.30 | 14 | 33.43 | ↓1.28 |
7 | P53505 | Actin, cytoplasmic type 5g | 41849.89/5.30 | 14 | 33.43 | ↓1.13 |
8 | P53505 | Actin, cytoplasmic type 5g | 41849.89/5.30 | 14 | 33.43 | - |
9 | P81947 | Tubulin alpha-1B chain | 50151.63/4.49 | 60 | 59.56 | ↑2.20* |
10 | P02548 | Neurofilament light polypeptide | 62514.51/4.58 | 9 | 35.48 | - |
11 | P55072 | Transitional endoplasmic reticulum ATPase | 89190.61/5.14 | 26 | 31.89 | - |
12 | P38646 | Stress-70 protein, mitochondrial | 68759.00/5.44 | 201 | 86.78 | ↑3.65** |
13 | P10809 | 60 kDa heat shock protein, mitochondrialg | 57962.86/5.24 | 37 | 49.04 | - |
14 | P10809 | 60 kDa heat shock protein, mitochondrialg | 57962.86/5.24 | 54 | 20.93 | - |
15 | P02680 | Fibrinogen gamma chain | 50632.74/5.62 | 8 | 20.37 | - |
16 | P14639 http://www.uniprot.org/uniprot/P14639 | Serum albumin | 69188.00/5.80 | 24 | 24.34 | ↓1.11 |
17 | Q8C196 | Carbamoyl-phosphate synthase [ammonia], mitochondrialg | 160307.8/6.09 | 383 | 88.05 | ↑1.50 |
18 | Q8C196 | Carbamoyl-phosphate synthase [ammonia], mitochondrialg | 160307.8/6.09 | 383 | 88.85 | ↑1.43* |
19 | Q8C196 | Carbamoyl-phosphate synthase [ammonia], mitochondrialg | 160307.8/6.09 | 383 | 88.85 | ↑1.11 |
20 | P05165 | Propionyl-CoA carboxylase alpha chain, mitochondrialg | 74246.99/6.16 | 25 | 29.76 | - |
21 | P05165 | Propionyl-CoA carboxylase alpha chain, mitochondrialg | 74246.99/6.16 | 29 | 33.84 | - |
22 | P05165 | Propionyl-CoA carboxylase alpha chain, mitochondrialg | 74246.99/6.16 | 29 | 33.84 | - |
23 | P05165 | Propionyl-CoA carboxylase alpha chain, mitochondrialg | 74246.99/6.16 | 29 | 33.84 | ↓1.25 |
24 | Q9ET01 | Glycogen phosphorylase, liver formg | 97331.88/6.65 | 25 | 22.57 | ↓1.29 |
25 | Q9ET01 | Glycogen phosphorylase, liver formg | 97331.88/6.65 | 26 | 31.65 | ↑1.45* |
26 | Q9ET01 | Glycogen phosphorylase, liver formg | 97331.88/6.65 | 26 | 31.65 | ↓1.29 |
27 | Q9ET01 | Glycogen phosphorylase, liver formg | 97331.88/6.65 | 39 | 45.27 | - |
28 | Q9ET01 | Glycogen phosphorylase, liver formg | 97331.88/6.65 | 81 | 66.41 | - |
29 | Q9ET01 | Glycogen phosphorylase, liver formg | 97331.88/6.65 | 39 | 45.27 | ↓2.38 |
30 | Q5MIB5 | Glycogen phosphorylase, liver formg | 97331.88/6.65 | 81 | 66.41 | ↓1.78 |
31 | Q8K0E8 | Fibrinogen beta chain | 54752.71/6.68 | 44 | 51.35 | ↓1.31 |
P11598 | Protein disulfide-isomerase A3 | 56623.37/5.88 | 18 | 32.28 | ↓1.31 | |
32 | P00439 | Phenylalanine-4-hydroxylase | 51862.09/6.15 | 8 | 14.73 | ↑1.89** |
33 | P49410 | Elongation factor Tu, mitochondrial | 45049.51/6.20 | 4 | 09.07 | ↑1.15 |
34 | Q3SZJ0 | Argininosuccinate lyase | 52743.07/6.04 | 13 | 20.26 | - |
35 | P54868-2 | Hydroxymethylglutaryl-CoA synthase, mitochondrialg | 52383.34/ 6.64 | 38 | 45.14 | - |
36 | P54868-2 | Hydroxymethylglutaryl-CoA synthase, mitochondrialg | 52383.34/ 6.64 | 21 | 38.52 | - |
37 | P54868-2 | Hydroxymethylglutaryl-CoA synthase, mitochondrialg | 56849.61/8.86 | 24 | 35.60 | - |
38 | Q64I00 | Glutamate dehydrogenase 2, mitochondrialg | 61385.85/8.63 | 124 | 99.00 | ↓1.28* |
39 40 | Q64I00 Q3SZB4 | Glutamate dehydrogenase2, mitochondrialg Medium-chain specific acyl-CoA dehydrogenase, mitochondrial | 61385.85/8.63 43586.83/7.02 | 212 8 | 99.00 16.14 | ↓1.11* ↑1.19 |
41 | Q5EA20 | 4-hydroxyphenylpyruvate dioxygenase | 44832.04/6.25 | 26 | 48.22 | ↑1.47** |
42 | P25708 | NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial | 48499.21/7.08 | 38 | 40.30 | - |
O09173 | Homogentisate 1,2-dioxygenaseg | 49959.92/6.85 | 9 | 11.91 | - | |
43 | O09173 | Homogentisate 1,2-dioxygenaseg | 49959.92/6.85 | 19 | 30.34 | ↑3.35** |
44 | Q8CI38 | Homogentisate 1, 2-dioxygenaseg | 49900.53/6.85 | 9 | 09.66 | ↑1.21 |
45 | P05089 | Arginase-1 | 35664.07/6.72 | 14 | 99.00 | - |
46 | A5PKH3 | Fumarylacetoacetaseg | 46024.69/6.49 | 20 | 25.30 | - |
47 | A5PKH3 | Fumarylacetoacetaseg | 46024.69/6.49 | 22 | 33.17 | ↑1.69 |
48 | P11708 | Malate dehydrogenase, cytoplasmic | 36299.97/6.15 | 28 | 52.56 | - |
49 | Q5FZI9 | Uricase | 34942.79/8.22 | 78 | 84.21 | - |
50 | Q3T165 | Prohibitin | 29804.10/5.57 | 125 | 99.00 | ↑1.18 |
51 | G3HAE3 | ATP synthase subunit alpha, mitochondrial | 31185.66/6.92 | 72 | 42.68 | - |
52 | Q76I81 | 40S ribosomal protein S12 | 14383.74/7.01 | 10 | 41.61 | ↑1.14 |
53 | P11757 | Hemoglobin subunit alpha | 15139.41/7.98 | 45 | 99.00 | ↓1.30* |
Accession number: Uniprot ID number of each protein identified.
MW/pI: Theoretical molecular weight and pI calculated by the Compute tool hosted in ExPASy.
MPN (matched peptide number): Number of unique peptides matched to the protein by TurboSequest search.
PC (peptide coverage, %): the combined length (number of amino acids) of matched peptides of the protein divided by the full length (number of amino acids) of the protein.
FC (fold change, H/A): The protein amount in hibernation (H) state was compared to that in active (A) state. ↑, up-regulation; ↓, down-regulation; –, no significant change. All fold changes were statistically significant with a P value <0.05; those marked with asterisks had a smaller P value: *, P<0.01; **, P<0.001.
Multiple spots: potential existence of post-translational modification forms of the same protein.