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. 2013 Apr 19;8(4):e62039. doi: 10.1371/journal.pone.0062039

Table 1. Comparison of protein expression levels in hibernation and active states of Myotis ricketti.

IDa Accession numberb Protein Identification (s) MW/pIc MPNd PCe (%) FCf(H/A)
1 Q6FG96 60S ribosomal protein P2, RPLP2 proteing 11657.85/4.38 9 48.70 -
2 Q6FG96 60S ribosomal protein P2, RPLP2 proteing 11657.85/4.38 9 48.70 ↓1.25
3 P02608 Myosin regulatory light chain 2, skeletal muscle isoform type 2 18895.34/4.79 24 99.00 ↓1.45*
4 P60661 Myosin, light polypeptide 6 16798.86/4.56 7 20.53 ↓1.29
5 P67936 Tropomyosin alpha-4 chain 28390.62/4.67 77 99.00 ↓1.72**
6 P53505 Actin, cytoplasmic type 5g 41849.89/5.30 14 33.43 ↓1.28
7 P53505 Actin, cytoplasmic type 5g 41849.89/5.30 14 33.43 ↓1.13
8 P53505 Actin, cytoplasmic type 5g 41849.89/5.30 14 33.43 -
9 P81947 Tubulin alpha-1B chain 50151.63/4.49 60 59.56 ↑2.20*
10 P02548 Neurofilament light polypeptide 62514.51/4.58 9 35.48 -
11 P55072 Transitional endoplasmic reticulum ATPase 89190.61/5.14 26 31.89 -
12 P38646 Stress-70 protein, mitochondrial 68759.00/5.44 201 86.78 ↑3.65**
13 P10809 60 kDa heat shock protein, mitochondrialg 57962.86/5.24 37 49.04 -
14 P10809 60 kDa heat shock protein, mitochondrialg 57962.86/5.24 54 20.93 -
15 P02680 Fibrinogen gamma chain 50632.74/5.62 8 20.37 -
16 P14639 http://www.uniprot.org/uniprot/P14639 Serum albumin 69188.00/5.80 24 24.34 ↓1.11
17 Q8C196 Carbamoyl-phosphate synthase [ammonia], mitochondrialg 160307.8/6.09 383 88.05 ↑1.50
18 Q8C196 Carbamoyl-phosphate synthase [ammonia], mitochondrialg 160307.8/6.09 383 88.85 ↑1.43*
19 Q8C196 Carbamoyl-phosphate synthase [ammonia], mitochondrialg 160307.8/6.09 383 88.85 ↑1.11
20 P05165 Propionyl-CoA carboxylase alpha chain, mitochondrialg 74246.99/6.16 25 29.76 -
21 P05165 Propionyl-CoA carboxylase alpha chain, mitochondrialg 74246.99/6.16 29 33.84 -
22 P05165 Propionyl-CoA carboxylase alpha chain, mitochondrialg 74246.99/6.16 29 33.84 -
23 P05165 Propionyl-CoA carboxylase alpha chain, mitochondrialg 74246.99/6.16 29 33.84 ↓1.25
24 Q9ET01 Glycogen phosphorylase, liver formg 97331.88/6.65 25 22.57 ↓1.29
25 Q9ET01 Glycogen phosphorylase, liver formg 97331.88/6.65 26 31.65 ↑1.45*
26 Q9ET01 Glycogen phosphorylase, liver formg 97331.88/6.65 26 31.65 ↓1.29
27 Q9ET01 Glycogen phosphorylase, liver formg 97331.88/6.65 39 45.27 -
28 Q9ET01 Glycogen phosphorylase, liver formg 97331.88/6.65 81 66.41 -
29 Q9ET01 Glycogen phosphorylase, liver formg 97331.88/6.65 39 45.27 ↓2.38
30 Q5MIB5 Glycogen phosphorylase, liver formg 97331.88/6.65 81 66.41 ↓1.78
31 Q8K0E8 Fibrinogen beta chain 54752.71/6.68 44 51.35 ↓1.31
P11598 Protein disulfide-isomerase A3 56623.37/5.88 18 32.28 ↓1.31
32 P00439 Phenylalanine-4-hydroxylase 51862.09/6.15 8 14.73 ↑1.89**
33 P49410 Elongation factor Tu, mitochondrial 45049.51/6.20 4 09.07 ↑1.15
34 Q3SZJ0 Argininosuccinate lyase 52743.07/6.04 13 20.26 -
35 P54868-2 Hydroxymethylglutaryl-CoA synthase, mitochondrialg 52383.34/ 6.64 38 45.14 -
36 P54868-2 Hydroxymethylglutaryl-CoA synthase, mitochondrialg 52383.34/ 6.64 21 38.52 -
37 P54868-2 Hydroxymethylglutaryl-CoA synthase, mitochondrialg 56849.61/8.86 24 35.60 -
38 Q64I00 Glutamate dehydrogenase 2, mitochondrialg 61385.85/8.63 124 99.00 ↓1.28*
39 40 Q64I00 Q3SZB4 Glutamate dehydrogenase2, mitochondrialg Medium-chain specific acyl-CoA dehydrogenase, mitochondrial 61385.85/8.63 43586.83/7.02 212 8 99.00 16.14 ↓1.11* ↑1.19
41 Q5EA20 4-hydroxyphenylpyruvate dioxygenase 44832.04/6.25 26 48.22 ↑1.47**
42 P25708 NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial 48499.21/7.08 38 40.30 -
O09173 Homogentisate 1,2-dioxygenaseg 49959.92/6.85 9 11.91 -
43 O09173 Homogentisate 1,2-dioxygenaseg 49959.92/6.85 19 30.34 ↑3.35**
44 Q8CI38 Homogentisate 1, 2-dioxygenaseg 49900.53/6.85 9 09.66 ↑1.21
45 P05089 Arginase-1 35664.07/6.72 14 99.00 -
46 A5PKH3 Fumarylacetoacetaseg 46024.69/6.49 20 25.30 -
47 A5PKH3 Fumarylacetoacetaseg 46024.69/6.49 22 33.17 ↑1.69
48 P11708 Malate dehydrogenase, cytoplasmic 36299.97/6.15 28 52.56 -
49 Q5FZI9 Uricase 34942.79/8.22 78 84.21 -
50 Q3T165 Prohibitin 29804.10/5.57 125 99.00 ↑1.18
51 G3HAE3 ATP synthase subunit alpha, mitochondrial 31185.66/6.92 72 42.68 -
52 Q76I81 40S ribosomal protein S12 14383.74/7.01 10 41.61 ↑1.14
53 P11757 Hemoglobin subunit alpha 15139.41/7.98 45 99.00 ↓1.30*
a

ID: spot number of proteins indicated in figures 1, S1, and S2.

b

Accession number: Uniprot ID number of each protein identified.

c

MW/pI: Theoretical molecular weight and pI calculated by the Compute tool hosted in ExPASy.

d

MPN (matched peptide number): Number of unique peptides matched to the protein by TurboSequest search.

e

PC (peptide coverage, %): the combined length (number of amino acids) of matched peptides of the protein divided by the full length (number of amino acids) of the protein.

f

FC (fold change, H/A): The protein amount in hibernation (H) state was compared to that in active (A) state. ↑, up-regulation; ↓, down-regulation; –, no significant change. All fold changes were statistically significant with a P value <0.05; those marked with asterisks had a smaller P value: *, P<0.01; **, P<0.001.

g

Multiple spots: potential existence of post-translational modification forms of the same protein.