Figure 2. Tissue specific expression of the lov transcripts in adults.

Semi-Q RT-PCR was used to detect lov transcripts in the bodies (soma), heads and gonads of male and female adults from a control (w¹¹¹⁸) strain and lov⁴⁷ and lov⁶⁶ mutant lines. Primers as indicated in Figure 1. In all cases, the fragments amplified span intron-exon boundaries to limit detection to mature transcripts. A fragment from the ubiquitous Actin mRNA (Actin at 57B) was amplified in parallel as the control. At least two separate RNA preparations for each tissue were used for transcript quantitation. The PCR fragment for transcript D was very close in size to the Actin PCR fragment. To quantitate this transcript, aliquots of separate transcript D and Actin PCR reactions for each RNA sample were run in parallel in separate gel lanes.