Figure 6. Roles for CRAC and KCa3.1 channels in microglia migration.

Primary cultured rat microglia were seeded on filters with 8 µm-diameter pores and placed in the upper insert of Transwell^TM chambers. After 24 h, transmigration was compared without (control) or with 100 µM UTP, in the lower chamber. The following antagonists were added to the microglia-containing upper well: the P₂Y receptor antagonist, 100 µM suramin; blockers of CRAC/Orai1 (50 µM 2-APB, 10 µM BTP2), KCa3.1 (1 µM TRAM-34) or SK1–SK3 (100 nM apamin). For each treatment, cell counts were summed from 5 random fields of view at 20× magnification. The number of separate cell cultures is indicated on each bar. Statistical differences between control and UTP-treated cells (^### p<0.001), and for UTP-treated cells with or without antagonists (***p<0.001) were determined by 1-way ANOVA with Tukey's post hoc test.