Figure 4. A neurotensin receptor antagonist SR142948 dose-dependently decreased wakefulness time and duration during the lights on period in wild-type mice, but not in orexin neuron-ablated mice.

Hourly amounts (A, B) and average episode duration (C, D) of awake, non-REM, and REM sleep states (mean±SE) plotted over 4 hr after administration of saline (dotted line) or SR142948 (solid line) in wild-type (n = 27, 14 and 7 for saline, SR 1 mg/kg, and 3 mg/kg, respectively) (A, C) and orexin/ataxin-3 mice (n = 14, 14 and 7 for saline, SR 1 mg/kg, and 3 mg/kg, respectively) (B, D). Data for the dark phase are displayed in shaded panels. SR142948 was administered at the start of light or dark periods (8:45 or 20:45).