Table 1. The information of SNPs in ATP2B2 and primers of PCR and unextended primers for Sequenom SNP genotyping.
SNP | Location | 2nd-PCR | 1st-PCR | UEP_SEQ |
rs35678 | Exon | ACGTTGGATGAACGAGGACGTGGAGGAGAT | ACGTTGGATGTGTCTGGATCCGATTCAGGC | GTGTAGGAGATCGACCACGC |
rs241509 | Intron | ACGTTGGATGCGTGATTGTGGCCTTCACAG | ACGTTGGATGTCCCCCAGGCTTTGGTGTG | ACTTCATCACGCAGGTGGGTCC |
rs3774180 | Intron | ACGTTGGATGAAACTGAGGCCATCTGTGAG | ACGTTGGATGACTCAAGTGACACAGCAGAC | GTGGCAGGGGCAGAA |
rs3774179 | Intron | ACGTTGGATGTTCCTTCAGTCAGGGCATCC | ACGTTGGATGGTGAGACAGTTTATGACTCC | GGGGATCCTGTTGGCTCACCCCTGGG |
rs2278556 | Intron | ACGTTGGATGAAAATCTGAGGCAAGTACGG | ACGTTGGATGGTTACGTGCCTATCATCCAG | ACGGCATAATGTTAAGATG |
SNP, single nucleotide polymorphism; PCR, polymerase chain reaction; UEP, unextended primer.