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. 2013 Apr 19;8(4):e61423. doi: 10.1371/journal.pone.0061423

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© 2013 Fu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. qRT-PCR of BMP2 expression in BMSCs from SD rats treated with varying concentrations of TSA. B. qRT-PCR of BMP2 expression in BMSCs from SD rats treated with varying concentrations of TSA followed by 1,25(OH)₂D₃. C. qRT-PCR of BMP2 expression in BMSCs from GHS rats treated with varying concentrations of TSA. D. qRT-PCR of BMP2 expression in BMSCs from GHS rats treated with varying concentrations of TSA followed by 1,25(OH)₂D₃. E. qRT-PCR of BMP2 expression in UMR-106 cells treated with varying concentrations of TSA. F. qRT-PCR of BMP2 expression in UMR-106 cells treated with varying concentrations of TSA followed by 1,25(OH)₂D₃. G. Antibody against acetyl-histone H3 was used to do ChIP assay on chromatin extracted from UMR-106 cells which were incubated with 1,25(OH)₂D₃ (together with or without TSA) or vehicle. The normal IgG was used as a negative control. Primer pairs designed for BMP2 promoter region C were used in the PCR. (*, P<0.05; **, P<0.01).