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. 2013 Apr 19;8(4):e61240. doi: 10.1371/journal.pone.0061240

Figure 2. Quantitative RT-PCR analysis of PMi for ribosomal protein genes in wild-type and hog1 or cbc1 mutants.

Figure 2

(A) A decrease in ribosomal protein (RP) pre-mRNAs in response to osmotic stress. The behaviour of transcripts RPL30, RPL28, RPL33A, RPL33B, RPL34A and YDR367W across a time course lasting 5, 10 and 15 minutes after treatment with 0.4 M NaCl was examined by quantitative RT-PCR using specific primers to intron regions and exon regions. The PMi value of the comparison made between stressed (0.4 M NaCl, 15 min) and non-stressed cells per gene, obtained by quantitative RT-PCR, is found in parentheses. Data and error bars represent the average and standard deviation of 3 independent experiments. (B) Representation of PMi for the wild-type, hog1 mutant and cbc1 mutant cells treated with 0.4 M NaCl for 15 minutes in relation to untreated cells. The intron and exon levels of the transcripts RPL30, RPL28, RPL37A, RPS13, RPL33A, RPL34A and RPL33B were examined by quantitative RT-PCR using specific primers to those regions. Data and errors bars represent the average and standard deviation of 3 independent experiments.