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. 2013 Apr 19;8(4):e61240. doi: 10.1371/journal.pone.0061240

Figure 4. Upf1 and exonuclease Xrn1 contribute to the decrease in ribosomal protein pre-mRNA during osmotic stress.

Figure 4

(A) The total and pre-mRNA relative levels are shown for the non-stressed cells of a wild-type strain and strains with deletions in RRP6, XRN1 or UPF1. (B) Representation of PMi in the cells treated with 0.4 M NaCl for 15 minutes in relation to untreated cells for the wild-type strain and the same mutant strains as in (A). The intron and exon levels of the transcripts RPL30, RPL28, RPL37A, RPS13, RPL33A, RPL34A, RPL33B and YDR367W were examined by quantitative RT-PCR using specific primers to those regions. Data and errors bars represent the average and standard deviation of 3 independent experiments.