Figure 9.

The TNF-α-induced actin remodeling occurs through a Cdc42- and PLC-γ1-dependent mechanism. Cells were transfected with Myc-tagged dominant negative Cdc42(T17N) (A) or preincubated with U73122 (B) and then incubated with TNF-α for 15 min. Transfected cells were identified by double immunofluorescence as described in MATERIALS AND METHODS. Cells expressing Myc-tagged Cdc42(T17N) are indicated by an arrowhead; arrows show untransfected cells. Bar, 30 μm. Similar results were obtained in three independent experiments.