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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Mol Cancer Res. 2013 Feb 1;11(4):393–404. doi: 10.1158/1541-7786.MCR-12-0542

Figure 2.

Figure 2

Both transient and stable HER2 overexpression in MCF7 breast cancer cells increases Fn14 protein expression. (A) MCF7 cells were transiently transfected with the indicated amount of a HER2 expression plasmid. Cells were harvested at 24 h post-transfection and Fn14, HER2 and tubulin expression analyzed by Western blotting. (B and C) MCF7 and MCF7/HER2 cells were analyzed for HER2, HER3, p-HER2, p-HER3, Fn14 and tubulin levels by Western blotting. (D) MCF7 and MCF7/HER2 cells were subjected to FACS analysis using an anti-Fn14 mAb (MCF7 cells: dark dashed line; MCF7/HER2 cells: dark solid line) or isotype control IgG (MCF7 cells: light dashed line; MCF7/HER2 cells: light dotted line). (E) MCF7 and MCF7/HER2-18 cells were analyzed for Fn14, HER2 and tubulin expression by Western blotting.