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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Mol Cancer Res. 2013 Jan 29;11(4):360–369. doi: 10.1158/1541-7786.MCR-12-0661

Figure 3. The association of NF-YA and its co-activator p300 with the human EZH2 gene promoter is enhanced in human EOC cells compared with normal HOSE cells.

Figure 3

(A) Diagram of the human EZH2 gene promoter with indicated positions of primers used for proximal (−64/+141) and distal (−875/−568) regions of the human EZH2 gene promoter in ChIP analysis. (B) Normal HOSE cells or indicated EOC cell lines were subjected to ChIP analysis using antibodies specific to NF-YA or p300, respectively. An isotype matched IgG was used as a negative control, and antibody against core histone H3 was used as a positive control. After ChIP analysis, the distal and proximal regions of the human EZH2 gene promoter were subjected to PCR amplification using primers detailed in materials and methods and as diagramed in (A). (C) Same as (B) but using an antibody against acetylated histone H3.