Figure 2.

Anti-Nop1 antibody staining shows enrichment of old cells in elutriated samples. Young dna2-1 and wild-type cells (A and B) and old dna2-1 and wild-type cells (C and D) isolated with the elutriation centrifuge were stained with DAPI (dark blue) and with anti-Nop1 antibody (green). The cells shown are representative of >75% of the cells observed in the elutriation sample. The old and young cells on each panel are photographed at the same magnification. Therefore, the difference in size of the images between young and old cells and between wild type and mutant are representative of the age and of the strain.