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. 2004 Mar;15(3):1297–1312. doi: 10.1091/mbc.E03-10-0742

Figure 9.

Figure 9.

Variation of expression of the genes involved in the RAD52 recombinational pathway determined by real-time RT-PCR confirms the microarray results. (A) Eight genes shown as being significantly up-regulated in the old wild-type cells by using microarray experiments are also significantly up-regulated in the RTPCR experiments. RT-PCR shows that RAD50, which has been filtered out in the microarray experiments, is also significantly up-regulated in the old wild-type cells. (B) In the dna2-1 old cells, eight genes that we found down-regulated with the microarray experiments are also down-regulated in old cells by using the RT-PCR technique. RAD51, the only gene belonging to the recombinational pathway found as being up-regulated in the old dna2-1 cells by using the microarray technique is also found up-regulated in the old dna2-1 cells by using RT-PCR. We used YMR118c, a succinate deshydrogenase, and CTS1, an endochitinase, as additional comparisons to verify correspondence between the dna2-1 microarray and RTPCR experiments.