Table 2.
Antibodies used in this study.
| Antigen | Species | Company (cat#) | Dilution (IF; IB) | Block (IB) |
|---|---|---|---|---|
| Actin, native | G | SCBT (Ac-1616) | ND; 1:3000 | NFDM |
| Cytokeratin 7, native | M | Invitrogen (18-00234) | 1:300; 1:3000 | NFDM |
| Cytokeratin 18, native | M | Invitrogen (18-0158Z) | 1:100; 1:1000 | NFDM |
| Cytokeratin 18, cleaved | M | Roche (12140322001) | 1:100; 1:1000 | NFDM |
| Caspase 8, cleaved | Rb | CST (9496) | 1:100; 1:1000 | BSA |
| E-cadherin, native | Rb | Abcam (ab40772) | 1:300; 1:3000 | NFDM |
| E-cadherin, native | M | Invitrogen (18-0223) | 1:100; ND | ND |
| hCG | Rb | Gift from Irv Boime | 1:100; ND | ND |
| Ki67 (MIB1) | M | Dako (M7240) | 1:100; ND | ND |
| PARP1, cleaved | Rb | CST (9541) | 1:100; 1:1000 | NFDM |
| Anti-rabbit, Alexa 488 | G | Invitrogen (A-21206) | 1:200; NA | NA |
| Anti-mouse, Alexa 555 | G | Invitrogen (A-11030) | 1:200; NA | NA |
G, goat; M, mouse; Rb, rabbit; NA, not applicable; ND, not done; CST, Cell Signaling Technology; SCBT, Santa Cruz Biotechnology, Inc.; IF, immunofluorescence; IB, immunoblotting. Block, indicates if 5% BSA or 5% non-fat dry milk (NFDM) in 1× PBS was used for blocking before immunoblotting. For immunofluorescence, 5% BSA was used for blocking.