Skip to main content
. 2004 Mar;15(3):1313–1323. doi: 10.1091/mbc.E03-02-0120

Figure 3.

Figure 3.

CD8-CLN3loop chimeras in NRK cells. The CD8-CLN3loop chimeras were expressed in stably transfected NRK cells and localized by confocal microscopy after fixation and permeabilization with methanol and double labeling with antibodies to CD8α (A and D) and lgp120 (B and E). The chimera containing the wt CLN3 loop (A) showed extensive overlap with with lgp120 (B). Arrows point to some of many structures that are positive for both proteins; merged images shown in C. The CD8-CLN3loopLI(253-4)AA chimera was detected at the plasma membrane (D) and showed no colocalization with lgp120 (E); merged images shown in F. Bar, 10 μm.