Table II.
Tm Data of IgG1 Antibody A and IgG4 Antibody B Fab Domains and Their Fab Fragments with the Same Variable Region
| Sample | Fab domain Tm (°C) |
|---|---|
| Antibody A IgG1 | 77.8 ± 0.2 |
| Fab A IgG1 Fab | 77.4 ± 0.6 |
| Antibody B IgG4 | 73.2 ± 0.3 |
| Fab B IgG4 | 73.7 ± 0.4 |
Antibody B IgG4 and Fab B IgG4 tested in 50 mM sodium phosphate, 300 mM sodium chloride, 250 mM imidazole, pH 8. Antibody A IgG1 and Fab A IgG1 tested in PBS pH 7.4. Tm Results demonstrate IgG1 and IgG4 Fab domain thermal stability is not influenced by the presence of the lower hinge region or Fc domain.