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. 2012 Jul 3;21(9):1323–1333. doi: 10.1002/pro.2119

Figure 1.

Figure 1

SDS-PAGE analysis of recombinant Txnip preparations. (A) Affinity purification under native conditions. Eluates were subjected to SDS-PAGE followed by Coomassie blue staining (left) and anti-Txnip immunoblotting (right). The arrow indicates His-Txnip, MBP-Txnip, or M2-Txnip. (B) Affinity purification under denaturing conditions. His-Txnip was expressed in E. coli. Eluates were subjected to SDS-PAGE followed by Coomassie blue staining (left) and anti-Txnip immunoblotting (right). The arrow indicates His-Txnip.