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Silibinin is not cytotoxic to RPE cells. Cell viability (%) was measured by the MTT assay. Treatment of cells with silibinin (1–500 μM) for 24 h did not affect cell viability (A) or cell morphology (B) Autophagy was detected by immunofluorescence assay (C). Treatment of silibinin inhibited hypoxia-induced LC3A fluorescenceintensity. Data are mean ± SE from three independent experiments.
