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. 2013 Jan 24;168(4):920–931. doi: 10.1111/j.1476-5381.2012.02227.x

Figure 3.

Figure 3

Association of HIF-1α and VHL proteins is prevented by silibinin. Formation of HIF-1α and VHL protein complexes was evaluated by immunoprecipitation and Western blotting. (A) RPE whole lysates used for immunoprecipitation were probed for their input HIF-1α and VHL levels. (B) Anti-VHL immune-complexes were analysed by blotting with anti-VHL and anti-HIF-1α antibodies. HIF-1α/VHL protein complex formation is increased by hypoxia and significantly reduced in the silibinin-pretreated group.