Figure 1.
Expression of ALDH2 in PK1 cells. Immunoblot analysis of cytosolic (Cyto) or mitochondrial (Mito) fractions from wild-type, PK1vector (Vector) and PK1ALDH2 cells (ALDH2); 20 μg of cytosolic or mitochondrial proteins was resolved on a 10% SDS-PAGE gel under reducing conditions, transferred to a PVDF membrane and probed with a rabbit polyclonal antibody to human ALDH2, and then to antibodies to the cytosolic and mitochondrial markers, β-actin and cytochrome c. Note that ALDH2 was detected in both the cytosol and mitochondria in PK1ALDH2 cells, whereas in wild-type or PK1vector cells, ALDH2 was only detected in the mitochondrial fraction.