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. 2013 Jan 24;168(4):1001–1014. doi: 10.1111/j.1476-5381.2012.02225.x

Figure 2.

Figure 2

Effects of lenti-PDE4D-shRNA on expression of PDE4 isoforms and variants in transfected HEK293 cells measured by quantitative real-time PCR. (A) 4DmiR and NC were well expressed in HEK293 cells as indicated by EGFP (green) observed under a fluorescence microscope. Scale bar = 100 μm. (B) The values of densitometric analysis were calculated by the 2−ΔΔCT method and normalized by the level of GAPDH. The mean ± SEM of three independent experiments is shown; *P < 0.05, **P < 0.01 versus corresponding NC.