Figure 4.
Effects of 4DmiR and/or rolipram (1.25 mg·kg−1) on the level of cAMP, phosphorylation of CREB, and expression of BDNF in the prefrontal cortices of mice. The prefrontal cortical tissues of 3 mm in diameter around the injection site were dissected for cAMP and Western-blotting assays. (A) Rolipram and/or 4DmiR treatment increased cAMP. (B, C) Rolipram and/or 4DmiR treatment increased pCREB (B) and BDNF (C). Lower panels are representative immunoblots detected by Western-blotting and upper panels are the corresponding quantifications. The values of densitometric analysis were normalized by the level of total CREB and β-actin, respectively. Values shown are means ± SEM; n = 4–5; *P < 0.05, **P < 0.01 versus the control (NC + Veh).