Table 1.

Primer sequences and optimal annealing temperatures used for SYBR green-based quantitative real-time polymerase chain reaction

Gene	Accession		Primer sequence (5′-3′)	Ta (°C)
PDE4A	AF208021	Fwd	ACACTGTCAGAGGAGACATGCCA	60.0
		Rev	ACCTGGTTTCCTGACCTGCTCATT
PDE4B	AF208023	Fwd	ATTTCCAGGCCAACCACACTACCT	65.0
		Rev	ATGAAGTACCAGTCCCGACGAAGA
PDE4D	NM_011056	Fwd	AGGGTCTGGGCTGATTCTC	60.0
		Rev	GTTGATGGATGGTTGGTTGC
PDE4D3	NM_001113332	Fwd	CTCAGCAGACGACAAGCC	63.5
		Rev	CCGCAGGGATTTCACAAG
PDE4D4	AF031373	Fwd	CATTCAACTGTTCTTGTGG	64.5
		Rev	AATCTTGTCCTAGTTCTTGG
PDE4D5	EF102484	Fwd	AGCGAGGAAATCCGTTTCTCCCAA	60.0
		Rev	TCCCGCTGATGTGCCATTGT
GAPDH	GU214026	Fwd Rev	CGCTGAGTACGTCGTGGAG GAGGAGTGGGTGTCGCTGTT	60.0

T_a, optimal annealing temperature; Fwd, forward; Rev, reverse primers; PDE4, phosphodiesterase 4.