Figure 1.

Initial characterization of capsaicin and PDBu evoked CGRP release from rat spinal cord homogenate. (A and B) Concentration–response curves to capsaicin or PDBu were inhibited by the TRPV1 antagonist BCTC and by removal of extracellular Ca²⁺. (C) Responses to PDBu were inhibited by the PKC inhibitor Ro-31,3220. (D) The structural analogue of PDBu, 4α-PDD did not evoke significant CGRP release above basal. (E) Responses to PDBu were enhanced at 30°C and 37°C as compared with 21°C. Responses were calculated as a percentage of 0.3 μM capsaicin in buffer containing Ca²⁺. Each data point represents the mean release ± SEM from at least three separate experiments.