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AOΔ22 is less stable than WT AO in vivo. Methanol-induced pex3 cells, producing WT AO or AOΔ22 protein, were shifted to glucose media to fully repress AO synthesis. Samples were taken at the indicated time points after the shift and analyzed by Western blotting using anti-AO antibodies. The level of AOΔ22 protein decreased with time, whereas WT AO levels remained constant. Equal portions of the cultures were loaded per lane.
