Table 4.

Number of codon sites estimated to be under positive or purifying selection in the SLAC, FEL, REL, and PARRIS analyses (P ≤ 0.05 in all cases) using the Dialign-T (D) and MUSCLE (M) alignments. In cases where different replicates of the same analysis returned different number of sites under selection, the lowest (most conservative) number is shown. C1 indicates the number of sites agreed upon to be under positive selection by FEL for the two different alignments; C2 indicates the equivalent number for REL; C3, indicates the number of sites under positive selection that are agreed upon by both REL and FEL for both alignments (note that SLAC did not predict any sites under positive selection)

	Significant sites at P ≤ 0.05
Salivary protein	# Codons		Positive selection								Purifying selection
			SLAC		FEL		REL		PARRIS		SLAC		FEL		REL		PARRIS
	D	M	D	M	D	M	D	M	D	M	D	M	D	M	D	M	D	M
																			C1	C2	C3
Antistasin	209	182	0	0	1	0	4	0	No	No	21	22	44	39	103	209*	N/A	N/A	0	0	0
Bdellin	80	63	0	0	0	0	0	0	No	No	10	9	17	16	19	19	N/A	N/A	0	0	0
Decorsin	70	70	0	0	0	0	0	0	No	No	0	0	2	12	70*	70*	N/A	N/A	0	0	0
Destabilase	151	144	0	0	1	1	5	14	No	No	15	16	32	31	34	45	N/A	N/A	1	3	1
Eglin c	128	123	0	0	0	0	1	0	No	No	2	5	23	18	35	21	N/A	N/A	0	0	0
Ficolin	216	208	0	0	1	1	0	0	No	No	8	8	44	43	50	46	N/A	N/A	1	0	0
Hirudin	155	135	0	0	0	0	0	0	No	No	1	1	13	11	155*	9	N/A	N/A	0	0	0
Manillase	419	330	0	0	1	0	1	2	No	Yes	3	2	40	25	32	121	N/A	N/A	0	0	0
Saratin	223	200	0	0	2	4	0	7	No	No	11	15	22	21	23	72	N/A	N/A	2	0	0
Elastase inhibitor	371	279	0	0	0	0	0	0	No	No	1	2	32	22	3	1	N/A	N/A	0	0	0
C-type lectin	212	197	0	0	0	2	0	0	No	No	11	11	31	28	26	14	N/A	N/A	0	0	0

^*No rates with dN > dS were inferred, suggesting that all sites are under purifying selection