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. Author manuscript; available in PMC: 2013 Oct 18.
Published in final edited form as: Oncogene. 2012 Jun 18;32(16):2087–2095. doi: 10.1038/onc.2012.220

Figure 1. Identification of potential substrates of PTPN14 using a phospho-proteomic approach.

Figure 1

(A) Schematic diagram of a phospho-proteomic approach to identify potential substrates of PTPN14. Cell lysates were made from the parental SW480 CRC cells, stable clones expressing either a wild-type (WT) PTP domain or a PTPN14 D1079A trapping mutant PTP domain. Proteins were digested with trypsin and pY containing peptides were enriched by anti-pY antibody (pY100)-conjugated beads. Peptide mixtures were profiled by mass-spectrometry. (B) List of the top candidate substrates of PTPN14. ND, not detected.