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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Mol Cell Endocrinol. 2013 Mar 7;370(0):130–137. doi: 10.1016/j.mce.2013.02.017

Figure 1. A germ cell-free Leydig cell fraction can be readily obtained from the testes of a single mouse by Percoll gradient centrifugation.

Figure 1

Mechanically dispersed interstitial cells from the testes of individual Mek1f/f;Mek2−/−;Cre− mice of the indicated ages were fractionated on Percoll gradients as described in Materials and Methods. The cells migrating into the 37% or 53% Percoll solutions were collected, lysed and analyzed for VASA (a germ cell marker), 3βHSD (a Leydig cell marker) or GAPDH (loading control) using Western blots. DMRT-1, a marker for Sertoli cells and spermatogonia was also undetectable in the 53% Percoll fraction (data not shown). The results of a representative experiment are shown.