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. Author manuscript; available in PMC: 2013 Apr 21.
Published in final edited form as: Clin Cancer Res. 2009 Apr 7;15(8):2789–2796. doi: 10.1158/1078-0432.CCR-08-2589

Figure 2. Induction of WT1-specific IFN-γ production by T cells following Ad-tWT1 vaccination.

Figure 2

C57BL/6 mice were vaccinated via footpad injection with either 40 μl of saline or 2.6 × 1010 particles of the Ad-tWT1 vector. Mice (n=4/group) were sacrificed weekly and the splenocytes were harvested. T cells producing IFN-γ in response to stimulation with WT1 peptide mix, an HIV peptide mix (negative control), media alone or PMA + ionomycin (positive control) were measured in an ELISpot assay. The mean (+/- s.d.) number of spots (T cells secreting cytokine) is depicted at each time point. * p < 0.005 for the comparison of saline control versus each time point of Ad-tWT1 vaccination. ** p < 0.01 for the comparison of the mean number of spots at day 21 versus day 7.